2.43
1.65
1.98
0.73
1.88
1.81
2.43
2.2 Maddeyên standard ên ku di xêza kalibrkirinê ya belavbûna giraniya molekulî ya nisbî de têne bikar anîn: însulîn, mîkopeptîd, glîsîn-glîsîn-tîrozîn-arjînîn, glîsîn-glîsîn-glîsîn
3 Amûr û amûr
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27.5
Bi tevayî, rêjeya asîdên amînî di berhemên Sustar de ji ya di berhemên Zinpro de zêdetir e.
Beşa 8 Bandorên bikaranînê
Bandorên çavkaniyên cûda yên mîneralên şop li ser performansa hilberînê û kalîteya hêkan a mirîşkên hêkdanînê di dawiya serdema hêkdanînê de
Pêvajoya Hilberînê
Teknolojiya kelasyonê ya armanckirî
Teknolojiya emulsîfîkasyona birînê
Teknolojiya zuwakirin û sprekirina bi zextê
Teknolojiya sarkirin û zuwakirinê
Teknolojiya pêşkeftî ya kontrola jîngehê
Pêveka A: Rêbazên ji bo diyarkirina belavbûna giraniya molekulî ya nisbî ya peptîdan
Pejirandina standardê: GB/T 22492-2008
1 Prensîba Testê:
Ew bi kromatografiya parzûna jelê ya performansa bilind hate destnîşankirin. Ango, bi karanîna dagirtina poroz wekî qonaxa sabît, li ser bingeha cûdahiya di mezinahiya girseya molekulî ya nisbî ya pêkhateyên nimûneyê de ji bo veqetandinê, ku li girêdana peptîd a dirêjahiya pêlê ya vegirtina ultraviyole ya 220nm hatî tespît kirin, bi karanîna nermalava hilberandina daneyan a taybetî ji bo destnîşankirina belavbûna girseya molekulî ya nisbî bi kromatografiya parzûna jelê (ango, nermalava GPC), kromatogram û daneyên wan hatin hilberandin, hatin hesab kirin da ku mezinahiya girseya molekulî ya nisbî ya peptîda soyayê û rêjeya belavkirinê were bidestxistin.
2. Reagent
Ava ceribandinê divê li gorî taybetmendiya ava duyemîn a di GB/T6682 de be, karanîna reagentan, ji bilî bendên taybetî, ji hêla analîtîk ve paqij e.
2.1 Reagent ev in: asetonîtrîl (ji hêla kromatografî ve paqij), asîda trîfloroasetîk (ji hêla kromatografî ve paqij),
2.2 Maddeyên standard ên ku di xêza kalibrkirinê ya belavbûna giraniya molekulî ya nisbî de têne bikar anîn: însulîn, mîkopeptîd, glîsîn-glîsîn-tîrozîn-arjînîn, glîsîn-glîsîn-glîsîn
3 Amûr û amûr
3.1 Kromatografiya Şileya Performansa Bilind (HPLC): stasyoneke xebatê ya kromatografiyê an entegratorek bi detektorek UV û nermalava hilberandina daneyan a GPC.
3.2 Yekîneya parzûnkirin û paqijkirina valahiyê ya qonaxa mobîl.
3.3 Mîzana elektronîkî: nirxa pilekirî 0.000 1g.
4 Gavên Xebatê
4.1 Şert û mercên kromatografîk û ceribandinên adaptasyona pergalê (şert û mercên referansê)
- 4.1.1 Stûna kromatografiyê: TSKgelG2000swxl300 mm×7.8 mm (qûtreya hundirîn) an stûnên jel ên din ên heman celebê bi performansa wekhev ku ji bo destnîşankirina proteîn û peptîdan guncaw in.
- 4.1.2 Qonaxa mobîl: Asetonitrîl + av + asîda trîfloroasetîk = 20 + 80 + 0.1.
- 4.1.3 Dirêjahiya pêlê ya tespîtkirinê: 220 nm.
- 4.1.4 Leza herikînê: 0.5 mL/min.
- 4.1.5 Dema tespîtkirinê: 30 deqe.
- 4.1.6 Qebareya derzîkirina nimûneyê: 20μL.
- 4.1.7 Germahiya stûnê: germahiya odeyê.
- 4.1.8 Ji bo ku pergala kromatografiyê pêdiviyên tespîtkirinê bicîh bîne, hate destnîşankirin ku di bin şert û mercên kromatografiyê yên jorîn de, karîgeriya stûna kromatografiya jel, ango hejmara teorîk a plakeyan (N), ne kêmtir ji 10000 be ku li ser bingeha lûtkeyên standarda sêpeptîdê (Glîsîn-Glîsîn-Glîsîn) hatiye hesabkirin.
- 4.2 Hilberîna xêzên standard ên giraniya molekulî ya nisbî
- Çareseriyên standard ên peptîdên giraniya molekulî ya nisbî yên jorîn ên cuda bi rêjeya giraniya 1 mg/mL bi hevahengkirina qonaxa mobîl hatin amadekirin, bi rêjeyek diyarkirî hatin tevlihevkirin, û dûv re bi nav parzûnek qonaxa organîk bi mezinahiya porê 0.2 μm~0.5 μm hatin fîltrekirin û di nav nimûneyê de hatin derzîkirin, û dûv re kromatogramên standardan hatin bidestxistin. Xêzên kalibrkirina giraniya molekulî ya nisbî û hevkêşeyên wan bi xêzkirina logarîtma giraniya molekulî ya nisbî li hember dema ragirtinê an jî bi regresyona xêzikî hatin bidestxistin.
4.3 Dermankirina nimûneyê
10 mg ji nimûneyê bi awayekî rast di firaxeke volûmetrîk a 10 mL de bipîvin, hinekî qonaxa mobîl lê zêde bikin, bi ultrasonîk 10 deqîqeyan bihejînin, da ku nimûne bi tevahî bihele û tevlihev bibe, bi qonaxa mobîl heta pîvanê were şilkirin, û dûv re bi nav parzûneke qonaxa organîk a bi mezinahiya porên 0.2μm~0.5μm were parzûnkirin, û parzûn li gorî şert û mercên kromatografîk ên di A.4.1 de hate analîzkirin.
- 5. Hesabkirina belavbûna giraniya molekulî ya nisbî
- Piştî analîzkirina çareseriya nimûneyê ya ku di 4.3 de di bin şert û mercên kromatografiyê yên 4.1 de hatiye amadekirin, girseya molekulî ya nisbî ya nimûneyê û rêjeya belavbûna wê dikare bi cîhgirtina daneyên kromatografiyê yên nimûneyê di nav xêza kalibrasyonê 4.2 de bi nermalava hilberandina daneyan a GPC were bidestxistin. Belavbûna girseyên molekulî yên nisbî yên peptîdên cûda dikare bi rêbaza normalîzekirina qada lûtkeyê, li gorî formula: X=A/A tevahî × 100 were hesibandin.
- Di formulê de: X - Rêjeya girseyî ya peptîdek giraniya molekulî ya nisbî di tevahiya peptîda di nimûneyê de, %;
- A - Rûbera lûtkeyê ya peptîdek giraniya molekulî ya nisbî;
- Tevahî A - berhevoka rûberên lûtkeyê yên her peptîdek giraniya molekulî ya nisbî, ku heta yek reqema dehekî tê hesibandin.
- 6 Dubarekirin
- Cudahiya mutleq di navbera du diyarkirinên serbixwe yên ku di bin şert û mercên dubarekirinê de hatine bidestxistin, divê ji %15ê navînîya aritmetîkî ya her du diyarkirinan derbas nebe.
- Pêveka B: Rêbazên ji bo Tesbîtkirina Asîdên Amînî yên Azad
- Pejirandina standardê: Q/320205 KAVN05-2016
- 1.2 Reagent û materyal
- Asîda asetîk a glacial: bi awayekî analîtîk paqij
- Asîda perklorik: 0.0500 mol/L
- Nîşander: Nîşandera binefşî ya krîstalî %0.1 (asîda asetîk a qeşayî)
- 2. Destnîşankirina asîdên amînî yên azad
Nimûne di 80°C de bo saetekê hatin hişkkirin.
Nimûneyê têxin konteynirek hişk da ku bi awayekî xwezayî sar bibe heta germahiya odeyê an jî heta germahiyek bikêrhatî sar bibe.Bi qasî 0,1 g nimûneyê (rastbûna 0,001 g) têxin nav firaxek konîkî ya hişk a 250 mL.Ji bo ku nimûne şilbûna derdorê nekişîne, zû ber bi gava din ve biçin.25 mL asîda asetîk a glacial lê zêde bikin û herî zêde 5 deqîqeyan baş tevlihev bikin.2 dilopên nîşana binefşî ya krîstal lê zêde bikinBi çareseriya tîtrasyonê ya standard a 0.0500 mol / L (±0.001) a asîda perklorik tîtrat bikin heta ku çareserî ji binefşî derbasî xala dawî bibe.
Hejmarê çareseriya standard a ku tê vexwarin tomar bikin.
- Testa vala di heman demê de pêk bînin.
- 3. Hesabkirin û encam
- Naveroka asîda amînî ya azad X di reagentê de wekî rêjeya girseyî (%) tê îfadekirin û li gorî formula jêrîn tê hesibandin: X = C × (V1-V0) × 0.1445/M × 100%, di formula jêrîn de:
- C - Têkeliya çareseriya asîda perklorik a standard bi mol di lîtreyekê de (mol/L)
- V1 - Qebareya ku ji bo tîtrîkirina nimûneyan bi çareseriya asîda perklorik a standard tê bikar anîn, bi milîlître (mL).
- Vo - Qebareya ku ji bo tîtrîsyona vala bi çareseriya asîda perklorik a standard tê bikar anîn, bi milîlîtreyan (mL);
M - Giraniya nimûneyê, bi gram (g).
| 0.1445: Giraniya navînî ya asîdên amînî wekhevî 1.00 mL çareseriya asîda perklorîk a standard [c (HClO4) = 1.000 mol / L]. | 4.2.3 Çareseriya tîtrîasyona standard a sulfata seryûmê: rêjeya c [Ce(SO4)2] = 0.1 mol/L, li gorî GB/T601 hatiye amadekirin. | |
| Pejirandina standardan: Q/70920556 71-2024 | 1. Prensîba diyarkirinê (Fe wek mînak) | Kompleksên hesinê asîdên amînî di etanola bêav de pir kêm dihelin û îyonên metalên azad di etanola bêav de dihelin, cudahiya di çareserbûnê de di navbera herduyan de di etanola bêav de ji bo destnîşankirina rêjeya kelasyonê ya kompleksên hesinê asîdên amînî hate bikar anîn. |
| Di formulê de: V1 - qebareya çareseriya standard a sulfata ceriumê ya ku ji bo tîtrîkirina çareseriya ceribandinê tê xerckirin, mL; | Etanolê bê hîd; mayî wekî xala 4.5.2 di GB/T 27983-2011 de ye. | 3. Gavên analîzê |
| Du ceribandin bi hev re bikin. 0.1 g ji nimûneya ku di 103 ± 2℃ de ji bo 1 saetê hişk bûye, bi rastbûna 0.0001 g bipîvin, 100 mL etanolê bêav lê zêde bikin da ku bihele, parzûn bikin, bermayiya parzûnê bi kêmî ve sê caran bi 100 mL etanolê bêav were şuştin, paşê bermayiyê veguhezînin firaxek konîk a 250 mL, li gorî xala 4.5.3 ya GB/T27983-2011 10 mL çareseriya asîda sulfurîk lê zêde bikin, û dûv re li gorî xala 4.5.3 ya "Ji bo bihele germ bikin û dûv re bihêlin sar bibe" ya GB/T27983-2011 gavên jêrîn pêk bînin. Testa vala di heman demê de pêk bînin. | 4. Destnîşankirina naveroka tevahî ya hesin | 4.1 Prensîba diyarkirinê wekî xala 4.4.1 a GB/T 21996-2008 e. |
4.2. Reagent û Çareserî
| 4.2.1 Asîda tevlihev: 150 mL asîda sulfurîk û 150 mL asîda fosforîk li 700 mL avê zêde bikin û baş tevlihev bikin. | 4.2.2 Çareseriya nîşander a sodyûm dîfenîlamîn sulfonat: 5g/L, li gorî GB/T603 hatiye amadekirin. | 4.2.3 Çareseriya tîtrîasyona standard a sulfata seryûmê: rêjeya c [Ce(SO4)2] = 0.1 mol/L, li gorî GB/T601 hatiye amadekirin. | |
| 4.3 Gavên analîzê | Du ceribandin bi hev re bikin. 0.1 g nimûneyê bi rastbûna 020001 g bipîvin, têxin nav firaxek konîk a 250 mL, 10 mL asîda tevlihev lê zêde bikin, piştî helandinê, 30 ml av û 4 dilop çareseriya nîşaneya sodyûm dîanîlîn sulfonat lê zêde bikin, û dûv re li gorî xala 4.4.2 di GB/T21996-2008 de gavên jêrîn pêk bînin. Di heman demê de ceribandina vala jî pêk bînin. | 4.4 Nûnertiya encaman | Rêjeya tevahî ya hesinê X1 ya kompleksên hesinê yên asîdên amînî ji hêla rêjeya giran a hesin ve, nirxa ku bi % tê îfadekirin, li gorî formula (1) hate hesabkirin: |
| X1=(V-V0)×C×M×10-3×100 | V0 - çareseriya standard a sulfata ceriumê ku ji bo tîtrîkirina çareseriya vala tê xerckirin, mL; | V0 - çareseriya standard a sulfata ceriumê ku ji bo tîtrîkirina çareseriya vala tê xerckirin, mL; | C - Tewra rastîn a çareseriya standard a sulfata ceriumê, mol/L5. Hesabkirina naveroka hesin di kelatayan deNaveroka hesin X2 di kelatê de ji hêla rêjeya giran a hesin ve, nirxa ku bi % tê îfadekirin, li gorî formula jêrîn hate hesabkirin: x2 = ((V1-V2) × C × 0.05585)/m1 × 100 |
| Di formulê de: V1 - qebareya çareseriya standard a sulfata ceriumê ya ku ji bo tîtrîkirina çareseriya ceribandinê tê xerckirin, mL; | V2 - çareseriya standard a sulfata ceriumê ku ji bo tîtrîkirina çareseriya vala tê xerckirin, mL;nom1-Girseya nimûneyê, g. Navînîya aritmetîkî ya encamên diyarkirina paralel wekî encamên diyarkirinê bigirin, û cûdahiya mutleq a encamên diyarkirina paralel ji %0,3 zêdetir nebe. | 0.05585 - giraniya hesinê ferrous ku bi graman tê îfadekirin, wekhevî 1.00 mL çareseriya standard a sulfata seryûmê C[Ce(SO4)2.4H20] = 1.000 mol/L.nom1-Girseya nimûneyê, g. Navînîya aritmetîkî ya encamên diyarkirina paralel wekî encamên diyarkirinê bigirin, û cûdahiya mutleq a encamên diyarkirina paralel ji %0,3 zêdetir nebe. | 6. Hesabkirina rêjeya kelasyonêRêjeya kelasyonê X3, nirxa ku bi % tê îfadekirin, X3 = X2/X1 × 100Pêveka C: Rêbazên ji bo Diyarkirina Rêjeya Kelasyona Zinpro |
Pejirandina standardê: Q/320205 KAVNO7-2016
1. Reagent û materyal
a) Asîda asetîk a qeşayî: bi awayekî analîtîk saf; b) Asîda perklorik: 0.0500mol/L; c) Nîşander: Nîşandera binefşî ya krîstalî 0.1% (asîda asetîk a qeşayî)
2. Destnîşankirina asîdên amînî yên azad
٢.١ Nimûne di ٨٠°C de bo saetekê hatin hişkkirin.
٢.٢ Nimûneyê têxin konteynirek hişk da ku bi awayekî xwezayî sar bibe heta germahiya odeyê an jî heta germahiyek bikêrhatî sar bibe.
2.3 Nêzîkî 0.1 g nimûneyê (rastbûna 0.001 g) têxin nav firaxek konîkî ya hişk a 250 mL.
٢.٤ Ji bo ku nimûne şilbûna derdorê nekişîne, zû derbasî gava din bibin.
2.5 25 mL asîda asetîk a glacial lê zêde bikin û herî zêde 5 deqeyan baş tevlihev bikin.
2.6 2 dilop ji nîşana binefşî ya krîstal lê zêde bikin.
2.7 Bi çareseriya tîtrasyonê ya standard a 0.0500mol/L (±0.001) a asîda perklorîk tîtrat bikin heta ku çareserî ji binefşî bibe kesk, di 15 saniyan de bêyî ku rengê wê biguhere.
٢.٨ Qebareya çareseriya standard a ku hatiye vexwarin tomar bike.
٢.٩ Testa vala di heman demê de pêk bînin.
- 3. Hesabkirin û encam
- Katalanî
- Physicochemical parameters
V1 - Qebareya ku ji bo tîtrîkirina nimûneyan bi çareseriya asîda perklorik a standard tê bikar anîn, bi milîlître (mL).
Vo - Qebareya ku ji bo tîtrîsyona vala bi çareseriya asîda perklorik a standard tê bikar anîn, bi milîlîtreyan (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Navnîşan: Rêya Qingpu ya Hejmar 147, Bajarê Shouan, Wîlayeta Pujiang, Bajarê Chengdu, Wîlayeta Sichuan, Çîn
Telefon: 86-18880477902
Berhem
Mîneralên şopîner ên neorganîk
- Mîneralên şopîner ên organîk
- Swahîlîyî
- Xizmeta xwerû
- Girêdanên bilez
Profîla Şîrketê
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Gujaratî | Ji bo lêpirsînê bitikîne | © Mafê çapkirinê - 2010-2025: Hemû maf parastî ne. | Nexşeya Malperê LÊGERÎNA SERKEFTÎ Telefon |
| Tel | 86-18880477902 | Javanesî | E-name |
| 8618880477902 | Çînî | Fransî | |
| Bird | Çînî | Fransî | Almanî Îspanyolî |
| Aquatic animals | Japonî | Koreyî | Erebî Yewnanî |
| Tirkî | Îtalî | ||
| Ruminant animal g/head day | January 0.75 | Îndonezî Afrîkansî Swêdî |
Polandî
- Baskî
- Katalanî
- Physicochemical parameters
Hindî
Laoyî
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Şona
Bûlgarî
- Sebuano
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Xirwatî
Holandî
| Application object | Ûrdûyî Vîetnamî | Content in full-value feed (mg/kg) | Efficacy |
| Gujaratî | Haîtî | Hausa | Kînyarwanda Hmongî Macarî |
| Piglets and fattening pigs | Îgbo | Javanesî | Kanadayî Khmer Kurdî |
| Qirgizî | Latînî | ||
| Bird | 300～400 | 45～60 | Makedonî Malayî Malayalamî |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Norwêcî
- Peştûyî
- Appearance: brownish-yellow granules
- Physicochemical parameters
Sirbî
Sesotho
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Şona
Sindî
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Swahîlîyî
Tacîkî
Tamîlî
Teluguyî
Thayîkî
| Application object | Ûrdûyî Vîetnamî | Content in full-value feed (mg/kg) | Efficacy |
| Yîddîşî | Yorûbayî | Zulû | Kînyarwanda Oriyayî Tirkmenî |
| Uygurî | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
